Thermo Scientific says new spectrometric kit simplifies insulin bioanalysis

Thermo Scientific InsuQuant Mass Spectrometric Immunoassay Kit provides an innovative solution for highly sensitive pre-analytical protein purification.

Thermo Scientific says its recently-launched ready-to-use InsuQuant Mass Spectrometric Immunoassay Kit will streamline insulin bioanalysis procedures for biopharmaceutical researchers, as well as for workers in the sports anti-doping industry.

The kit incorporates all necessary reagents and consumables needed in the pre-analytical stage of the LC-MS workflow in a convenient, easy-to-use platform.

With the new product, researchers no longer need to make buffers and match reagents, thus saving resources and reducing the risk of human errors in complex protocols.

“Current protein purification methodologies require researchers to source their own reagents and buffers, which can result in complex, lengthy procedures that leave more room for error and inconsistencies during downstream analysis,” said Sam Bonfig, product manager for affinity consumables products, Thermo Fisher Scientific. “Our goal is to simplify our customers’ workflows, and our new kit does just that by providing an out-of-the-box solution containing insulin standards, elution solvents, an LC column, and all other components needed to run the pre-analytical affinity capture of insulin.”

A proprietary microcolumn technology enables the InsuQuant Mass Spectrometric Kit to make advanced detection of endogenous insulin and its exogenous analogues in the tip of a pipette, while also reducing background and improving reproducibility.

The kit is fully compatible with both the Thermo Scientific Versette automated liquid handler and the Finnpipette Novus i multichannel electronic pipette. Following sample preparation with the InsuQuant Mass Spectrometric Kit, users can achieve accurate, precise and sensitive separation on the Thermo Scientific Vanquish Flex Quaternary UHPLC system, and perform quantification of complex insulin analogues using the Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer.

The mass spectrometric immunoassay (MSIA) methodology has demonstrated pg/mL levels of detection while yielding simultaneous qualitative and quantitative data from complex biological matrices, making it well suited for use with plasma.

The kit will be on display at the annual American Society of Mass Spectrometry (ASMS) Conference from June 4-8 in the Thermo Fisher Hospitality Suite, White River Ballroom F, at the JW Marriott in Indianapolis, Indiana. For more information, click here.


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