Aurora Scientific calcium fluorescence system

The 950A calcium fluorescence system from Aurora Scientific enables physiology researchers to easily measure intracellular calcium along with tissue length, force, and sarcomere length. 

Powerful twitch-based software controls all instruments and collects and analyzes the data.  The entire system can be automated to perform entire experiments in a repeatable fashion.

Features include:

• Integrates with various Aurora Scientific systems to provide simultaneous acquisition of fluorescence photometry with length, force, and sarcomere length
• Controls all Aurora Scientific instruments plus light sources from major manufacturers (IonOptix HyperSwitch and Sutter Lambda DG-4).
• Complete fluorescence photometry system includes hardware and software
• Twitch based data acquisition and analysis ideal for cardiac studies, includes stimulator control
• System can be configured to work with any indicator fluorophore (BCECF, Fluo-3/4, Fura-2, Indo-1)
• Two operating modes for Fura-2 – interleaved and interpolated numerator
• Can calculate ratio or calcium (Ca2+) concentration at up to 1000 samples per second

The 955A FluoroTrack software controls the IonOptix fluorescence photometry equipment and collects the fluorescence signals from the photomultiplier.

Included components:

Included Components:

• 955A: FluoroTrack – Calcium Fluorescence Software
• 960A: FluoroDAQ Data Acquisition Controller
• 961A: HyperSwitch Dual Excitation Light Source
• 962A: Cell Framing Adapter
• 962B CFA Option D: Dual Emission
• 963A: Photomultiplier Subsystem
• 964-L Microscope Components – Leica
• 964-N Microscope Components – Nikon
• 964-O Microscope Components – Olympus
• 964-Z Microscope Components – Zeiss
• 965A Option S: 75W Xenon Light Source

Test protocols written using the 600A Protocol Editor control the entire experiment including the stimulator, high-speed length controller, force transducer, apparatus, fluorescence equipment and sarcomere length measurement.

Data collection from all of these instruments is stored in a single time-stamped file.  This integrated system allows a single computer to control and store data from many different instruments including those from Aurora Scientific, IonOptix and Sutter (DG-4 light source).